Some simple and rapid thin-layer chromatographic methods for clinical purposes
نویسندگان
چکیده
منابع مشابه
A rapid and simple thin-layer chromatographic method for amino acids in blood.
Simple and rapid thin-layer chromatographic methods, using heel or finger-tip blood, are describedthat are suitable for screening for inborn errors of amino acid metabolism. Serum is chromatographed without deproteinizing. A variation of this method utilizes blood impregnated on paper discs for chromatography. These methods are much faster, more sensitive, and provide better resolutionof amino ...
متن کاملSome simple thin-layer chromatographic systems for the separation of purines and purine nucleosides.
Studies on nucleic acid metabolism often require the analytical separation of nucleic acid derivatives and are facilitated by chromatographic methods which are rapid and convenient. Column chromatography on ion exchange resins (1)) paper chromatography (2)) and paper electrophoresis (3) are among the methods used routinely for such separations. Even though these techniques have been employed wi...
متن کاملThin-layer chromatographic technique for rapid detection of bacterial phospholipases.
Silica gel thin-layer chromatography was employed to detect lecithinase activity induced from bacterial resting cell preparations induced from bacterial resting cell preparations incubated at 37 C for 4 h in the presence of purified egg yolk lecithin. Bacillus subtilis, Bacillus cereus, Serratia marcescens, and Pseudomonas aeruginosa hydrolyzed lecithin with the formation of free fatty acids as...
متن کاملThin Layer Chromatographic Separation of Benzodiazepine Derivates
abstract: In this paper we describe the thin layer chromatographic separation of eight of the most frequently used benzodiazepine derivates (alprazolam, bromazepam, chlorazepate potassium, chlordiazepoxide, diazepam, nitrazepam, oxazepam) and their degradation products after acid hydrolysis. Our aim was not only to develop a simple, rapid and efficient method for their separation but also the o...
متن کاملA simple thin-layer-chromatographic method for detection of urinary homocystine.
For the detection of homocystine in urine specimens, a simple thin-layer chromatographic method was devised. Thirty ƒÊl of urine specimens without any prior treatment were applied onto a silica gel-G plate, and developed by ascending chromatography using a phenol water mixture (3: 1) containing 0.05 0 0 potassium cyanide. After developing and drying the plate, a slightly modified potassium iodo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1971
ISSN: 0306-3283
DOI: 10.1042/bj1220035p